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Bio-Rad
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Bio-Rad
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Seegene Technologies
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PANAGENE Inc
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PANAGENE Inc
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Pelco Products Inc
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Qiagen
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Riken Genesis Co Ltd
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Camlab Ltd
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PANAGENE Inc
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PANAGENE Inc
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PANAGENE Inc
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Image Search Results
Journal: BMC Cancer
Article Title: Exploratory biomarker analysis for treatment response in KRAS wild type metastatic colorectal cancer patients who received cetuximab plus irinotecan
doi: 10.1186/s12885-015-1759-y
Figure Lengend Snippet: Baseline characteristics of the patients enrolled in this study
Article Snippet: Peptide nucleic acid-locked nucleic acid (PNA–LNA) PCR clamp reactions were carried out using the PNA-ClampTM BRAF , and
Techniques: Mutagenesis
Journal: Biology Methods & Protocols
Article Title: Cationic copolymers that enhance wild-type-specific suppression in BNA-clamp PCR and preferentially increase the T m of fully matched complementary DNA and BNA strands
doi: 10.1093/biomethods/bpac009
Figure Lengend Snippet: Oligonucleotides used in this study
Article Snippet:
Techniques: Sequencing, Real-time Polymerase Chain Reaction
Journal: Biology Methods & Protocols
Article Title: Cationic copolymers that enhance wild-type-specific suppression in BNA-clamp PCR and preferentially increase the T m of fully matched complementary DNA and BNA strands
doi: 10.1093/biomethods/bpac009
Figure Lengend Snippet: Effects of representative copolymers (P17) on wild-type-specific suppression in BNA-clamp PCR to detect KRAS G13D mutation from genomic DNA. ( A ) Real-time PCR to amplify the KRAS gene encompassing the G13 codon was performed with genomic DNA templates (1.65 × 10 4 copies per reaction) of wild-type cells (HCC70) and mutant cells (MDA-MB-231) in the presence and absence of the BNA clamp and 0.025% (w/v) P17 polymer. ( B ) While keeping the total amount of the genomic DNA template at 1.65 × 10 4 copies per reaction, the proportion of DNA from the mutant cells was incrementally decreased to 1%, 0.1%, 0.01%, and 0% (i.e. 100% wild-type as a reference). The BNA clamp was included in both reactions. ( C ) Direct sequencing of PCR products from the reaction shown in B at 1%, 0.1%, and 0.01% mutant-to-wild-type ratio.
Article Snippet:
Techniques: Mutagenesis, Real-time Polymerase Chain Reaction, Polymer, Sequencing
Journal: Biology Methods & Protocols
Article Title: Cationic copolymers that enhance wild-type-specific suppression in BNA-clamp PCR and preferentially increase the T m of fully matched complementary DNA and BNA strands
doi: 10.1093/biomethods/bpac009
Figure Lengend Snippet: Effects of the co-polymers on threshold cycle ( C t ) of KRAS wild-type and G13D mutant amplification in BNA-clamp PCR
Article Snippet:
Techniques: Mutagenesis, Amplification, Polymer, Concentration Assay
Journal: Biology Methods & Protocols
Article Title: Cationic copolymers that enhance wild-type-specific suppression in BNA-clamp PCR and preferentially increase the T m of fully matched complementary DNA and BNA strands
doi: 10.1093/biomethods/bpac009
Figure Lengend Snippet: Melting curve analysis of 11-mer complementary strands encompassing the KRAS G13D mutation site. ( A ) Profiles from DNA–DNA strands with a perfect match (wild-type) and a single nucleotide mismatch (mutant) with the addition of P12. ( B ) Profile from the DNA–BNA strands equivalent to (A).
Article Snippet:
Techniques: Mutagenesis
Journal: Endocrine Pathology
Article Title: Identification of NIFTP-Specific mRNA Markers for Reliable Molecular Diagnosis of Thyroid Tumors
doi: 10.1007/s12022-023-09781-1
Figure Lengend Snippet: Baseline characteristics of patients in the discovery and validation populations
Article Snippet: All tumor samples utilized in this study were evaluated for the status of the
Techniques:
Journal: Journal of Pathology and Translational Medicine
Article Title: Highly prevalent BRAF V600E and low-frequency TERT promoter mutations underlie papillary thyroid carcinoma in Koreans
doi: 10.4132/jptm.2020.05.12
Figure Lengend Snippet: Baseline characteristics
Article Snippet: BRAF V600E mutation was analyzed using the real-time PCR clamping technology of
Techniques: Biomarker Discovery, Variant Assay, Mutagenesis
Journal: Journal of Pathology and Translational Medicine
Article Title: Highly prevalent BRAF V600E and low-frequency TERT promoter mutations underlie papillary thyroid carcinoma in Koreans
doi: 10.4132/jptm.2020.05.12
Figure Lengend Snippet: Association between TERT promoter alterations and clinicopathologic features in 724 consecutive patients with papillary thyroid carcinoma
Article Snippet: BRAF V600E mutation was analyzed using the real-time PCR clamping technology of
Techniques: Biomarker Discovery, Variant Assay, Mutagenesis
Journal: Journal of Pathology and Translational Medicine
Article Title: Highly prevalent BRAF V600E and low-frequency TERT promoter mutations underlie papillary thyroid carcinoma in Koreans
doi: 10.4132/jptm.2020.05.12
Figure Lengend Snippet: Clinicopathologic features of papillary thyroid carcinoma patients with TERT promoter alterations
Article Snippet: BRAF V600E mutation was analyzed using the real-time PCR clamping technology of
Techniques: Variant Assay
Journal: Journal of Pathology and Translational Medicine
Article Title: Highly prevalent BRAF V600E and low-frequency TERT promoter mutations underlie papillary thyroid carcinoma in Koreans
doi: 10.4132/jptm.2020.05.12
Figure Lengend Snippet: A pie chart depicting a portion of BRAF V600E and telomerase reverse transcriptase ( TERT ) promoter mutations (C228T and C250T) in relation to histologic variants of papillary thyroid carcinoma (n=724). The middle and inner circles show the frequency of BRAF V600E and TERT promoter mutations, respectively. The other variants included eight diffuse sclerosing, eight oncocytic, five solid, three hobnail, and one cribriform-morular variant. TCF, tall cell features.
Article Snippet: BRAF V600E mutation was analyzed using the real-time PCR clamping technology of
Techniques: Reverse Transcription, Variant Assay